A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

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Abstract Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in Customizable Bezels the United Arab Emirates (UAE).In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798.We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, and the assembly had a contig N50 of 18.

56 kb, L50 of three, and a BUSCO completeness score of 98.6% for 758 orthologous genes.Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms.Compared to five other Leggings Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.

598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains.These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.